The PNL - BDNF - IRES 2 - EGFP plasmid was identified to be correct.

构建的慢病毒载体质粒PNL -BDNF-IRES2-EGFP 经SalI和 BamHI 双酶切鉴定正确,三质粒共转染293T细胞 后荧光激发可见大量绿色荧光.

互联网

Both genes were cloned into GFP expression vector pIRES 2 EGFP and transfected into HeLa cells.

将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构.

互联网

The EGFP - GST solution after purification show bright kelly.

菌体超声裂解后上清液及其纯化产物在长波紫外线的照射下,发出明亮的绿色荧光.

互联网

RESULTS: The eukaryotic expression vector pIRES 2 - EGFP containing the truncated human AIF ( AIF ? ? 1 - 480 ) gene was successfully constructed.

结果: 成功构建了人截短型AIF ( AIF△1480 ) 基因的真核表达载体.

互联网

RESULTS: The eukaryotic expression vector pIRES 2 - EGFP containing the truncated human AIF ( AIF 1 - 480 ) gene was successfully constructed.

结果: 成功构建了人截短型AIF ( AIF1480 ) 基因的真核表达载体.

互联网