EWS - FLI 1 fusion transcripts were detected by nested RT - PCR.

EWS-FLI1融合基因检测方法采用嵌套式RT - PCR法.

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Conclusions 1 . The expression vector pS 2 - DTA contains the EWS - FLI - 1 binding sequence and the DTA gene sequence.

结论1、我们通过定向克隆的方法,成功地构建了含 EWS -FLI-1特异结合序列和白喉毒素A链基因的真核表达载体.

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After the PBMC were transfected by Ad EWS - FLI 1 , the EWS - FLI 1 mRNA was detected by ( RT - PCR . )

转染PBMC后,RT -PCR 证实有EWS -FLI1mRNA的 转录.

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The capabilities and capacities, of each wholesaler are identified through the EWS survey.

每个批发商的能力和能量已经批发商调查中确认.

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We found no significant difference in the sex ratio between EWS and CS ( P > 0.05 )

实验增温灌丛样地与对照间性比的差异不显著 ( P>0.05 ).

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