Method: The PML - RAR α fused protein in APL patients was stained by immuno - fluorescence antibody for PML.
方法: 采用免疫荧光技术检测APL患者PML-RARα融合蛋白.
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SDS - PAGE showed that the fused protein of interest has been specifically expressed as 61 kD.
所得菌体总蛋白经SDS -PAGE 分析,结果表明,在预期的61kD处 得到一条特异带,为AK( 1-3)cDNA与 载体固有基因DHFR-6хHis的重组蛋白表达产物.
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