BL 21 and an expected 14×103 fusion protein was isolated by SDS - PAGE.

重组原核表达质粒 pET28a -COP转化大肠杆菌BL21后,经IPTG诱导表达,获得一条约14×103的融合蛋白.

互联网

The fusion protein was purified through Nickel - affinity chromatography column.

用镍柱纯化融合蛋白.

互联网

Methods: The recombinant fusion protein MBP hEra was expressed in the E.

方法在大肠杆菌中,表达重组MBP?hEra融合蛋白.

互联网

Western - blot analysis with sera against CAV showed the fusion protein had good specificity.

在 Western -blot免疫 印迹分析中,3个融合蛋白与CAV阳性 血清均能发生特异性反应,表明它们都具有良好的反应性.

互联网

Mouse polyclonal antibody against GST - Norpeg fusion protein was prepared with purified GST - Norpeg fusion protein as immunogen.

以纯化的重组目的蛋白作为免疫原免疫小鼠,制备相应的多克隆抗体.

互联网

BVP 22 can mediate intercellular trafficking of fusion protein in vitro and in vivo.

在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭.

互联网

Conclusion: EGF - Ang fusion protein had definitely cytotoxicity to Hep 2 cells which over expressed EGFR in vitro.

结论: 融合蛋白EGF-Ang在体外对过度表达EGFR的Hep2细胞具有明显的杀伤作用.

互联网

The invention discloses a fusion protein, coded genes thereof and an application thereof.

本发明公开了一种融合蛋白及其编码基因与应用.

互联网

Results: The CAT gene was cloned correctly and its fusion protein was expressed in E . coli .

结果: 成功克隆了CAT的基因片段,并在 大肠杆菌 中得到其融合蛋白的表达.

互联网

The fusion protein could be detected by ELISA in COS 7 cell culture surpernatant.

重组真核表达载体CD5lneglKIR2DL1转染COS7细胞后,ELISA法检测细胞培养上清中有融合蛋白的表达.

互联网

Conclusion The GRA 7 gene may be expressed as a GST fusion protein in E . coli.

结论　GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达.

互联网

Conclusion: PC - 1 NF fusion protein may inhibit the proliferation, induce apoptosis and decrease ECM production of RMC.

结论PC-1NF融合蛋白能抑制大鼠系膜细胞增殖 、 诱导细胞凋亡及减少细胞外基质合成.

互联网

Cell density cultivation and high level expression of recombinant urease A fusion protein in Helicobacter pylori.

目的高密度培养重组细菌和高效表达重组幽门螺杆菌尿素酶A融合蛋白.

互联网

Objective To construct the GST fusion protein expression plasmid of cardiotrophin - 1 ( CT - 1 ) and tetanus toxin C frag - ( ment ( TTC ). )

目的构建心肌营养素 -1 ( CT -1 ) 和破伤风 外毒素 C片段 ( TTC ) 重组融合蛋白表达质粒.

互联网