Methods: The cell line transfected with E 1 A gene was treated by Epirubicin, Taxotere and 5 - fluorouracil respectively.

方法通过PEI -Fe3O4 纳米粒介导将E1A基因导入 人乳腺癌细胞系MCF-7,经G418筛选获得稳定表达E1A的转染细胞(MCF-7-E1A ).

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G 418 - resistant clones of Hela cells were obtained and confirmed as positive by RT - PCR.

重组质粒转染Hela细胞后,经 G418 筛选成功获得阳性克隆.

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