Results: HPV 16 E 7 was expressed efficiently in HEK - 293 and HeLa cells.

结果: 荧光显微镜下,转染后HEK-293细胞和HeLa细胞 表达绿色荧光蛋白.

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G 418 - resistant clones of Hela cells were obtained and confirmed as positive by RT - PCR.

重组质粒转染Hela细胞后,经 G418 筛选成功获得阳性克隆.

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Both genes were cloned into GFP expression vector pIRES 2 EGFP and transfected into HeLa cells.

将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构.

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