BL 21 and an expected 14×103 fusion protein was isolated by SDS - PAGE.

重组原核表达质粒 pET28a -COP转化大肠杆菌BL21后,经IPTG诱导表达,获得一条约14×103的融合蛋白.

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The cDNA was subcloned into prokaryotic expression vector pET 3 d and overexpressed in E . coli BL 21 ( DE 3 ).

将该cDNA插入原核表达载体pET3d并在 大肠杆菌 BL21 ( DE3 ) 中过量表达.

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The apr gene was expressed in E . coli BL 21 and showed its activities of protease.

apr基因在 大肠杆菌 BL21中获得表达,并表现出蛋白酶活性.

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