RT - PCR was used to determine GK and PEPCKgene expression ( corrected by ? ? - actin ) .

用RT -PCR 检测GK和 PEPCK mRNA表达水平的变化.

互联网

Results CnA α, CnA ? ? could be detected by RT - PCR but no CnA ? ?.

结果RT -PCR 可检测到CnAα 、 CnAβ,未检测到CnAγ.

互联网

RT - PCR was used to detect the eNOS mRNA and iNOS mRNA.

RT -PCR 分别检测内皮细胞eNOS、iNOS基因的相对表达量.

互联网

The expressions of bcl - x, bax and eNOS were measured by SQ - RT - PCR technique.

采用半定量逆转录聚合酶链反应(SQ-RT-PCR)法检测内皮细胞bcl-x 、 bax以及 eNOS表达.

互联网

Expression of TGF ? ? 1 mRNA was evaluated by RT PCR.

RT?PCR方法测定肺组织内TGF?β1的表达.

互联网

RT - PCR confirmed that AtT - 20 cells presented an endogenous expression of H _ 4 - receptor mRNA.

RT -PCR 结果证实,在AtT-20细胞中存在组胺H4受体mRNA的内源性表达.

互联网

Methods Reverse transcription - polymerase chain reaction ( RT - PCR ), and immunohistochemical method were used.

方法采用反转录 - 聚合酶链反应 ( RT -PCR ) 法和免疫组织化学方法.

互联网

Methods Plasma ETA was measured quantitively by RT PCR.

方法　应用RT-PCR技术检测外周血循环中ETA基因表达水平.

互联网

The iNOS mRNA levels was determined by using RT - PCR .

RT -PCR 方法测定诱导型一氧化氮合酶(iNOS)mRNA水平.

互联网

Expression of TGF ? ? 1 was evaluated by RT PCR technique.

逆转录聚合酶链反应(RT?PCR)方法测定肺组织内TGF?β1的表达.

互联网

EWS - FLI 1 fusion transcripts were detected by nested RT - PCR.

EWS-FLI1融合基因检测方法采用嵌套式RT - PCR法.

互联网

IC - RT - PCR and RT - PCR were more sensitive than DAS - ELISA.

从检测的可靠性来看,在春季所检测的8个无症品种中,RT -PCR 和IC-RT-PCR全部检测出了GLRaV一3,而DAS一ELISA仅 检测出其中的6个.

互联网

Norwalk virus antigen was detected by RT - PCR .

采用RT -PCR 检测诺瓦克病毒抗原.

互联网

It is confirmed by RT - PCR , Western blot that CAV 2 - Δ E 3 - Rgp can express Rgp antigen in MDCK cell.

通过RT -PCR 、 Westernblot等检测,表明该重组病毒能够表达Rgp抗原.

互联网

Conclusions Detection of target gene expression in paraffin - embedded tissues is feasible by RT - PCR or RQ - PCR .

结论应用RT-PCR或RQ -PCR 方法在石蜡包埋组织中检测目的基因的表达是可行的.

互联网