Methods Porcinecornealendotheliawereobtainedwiththeepitheliaservingas control , CEN - Agandcornealepithelialantigen ( CEP - Ag ) wereisolatedandpurifiedby ion - exchangechromatographyandaffinity chromatographywith 2 B 4.14.1 monoclonalantibody. TherabbitswereimmunizedwiththeresultantCEN - AgandCEP - Ag andtheirseracollectedto harvestaCEN - Abandanti - cornealepithelialantibody ( aCEP - Ab ) by affinitychromatography.

方法用猪角膜内皮做抗原,上皮做对照,经离子交换层析及2B4.14.1单克隆抗体亲和层析制得纯化的CEN-Ag 和角膜上皮抗原 ( cornealepithelialantigen, CEP-Ag ),用提纯的CEN-Ag和 CEP-Ag分别免疫家兔,取血清经亲和层析得到aCEN-Ab和抗角膜上皮抗原抗体(anti-cornealepithelialantibody, aCEP -Ab ).

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