Methods Reverse transcription - polymerase chain reaction ( RT - PCR ), and immunohistochemical method were used.
方法采用反转录 - 聚合酶链反应 ( RT -PCR ) 法和免疫组织化学方法.
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TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction ( RT - PCR )
用逆转录多聚酶联反应 ( RT -PCR ) 测定KCs中TLR4mRNA的表达.
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The gene expression of ET - 1、 ETAR 、 ETBR and ECE was evaluated by semi - quantitative reverse transcription polymerase chain response ( RT - PCR ).
采用半定量逆转录多聚酶链反应 ( RT -PCR ) 检测局部内皮素系统ET -1 、ETAR、ETBR及ECE的基因表达.
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Semi - quantitative reverse transcription - polymerase chain reaction ( RT - PCR ) was used to detect the level of MMP - 12 mRNA expression.
以 半定量 RT -PCR 法检测MMP -12 mRNA表达水平.
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The proto - oncogene c - fos mRNA expression was assessed using reverse transcription polymerase chain reaction ( RT - PCR ).
用逆转录聚合酶链式反应 ( RT -PCR ) 检测心肌细胞原癌基因c-fosmRNA的表达.
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Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction ( RT - PCR )
用反转录PCR ( RT-PCR ) 检测转染细胞ATF基因的表达.
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G proteins mRNA expressions were detected by the method of reverse transcription polymearse chain reaction ( RT - PCR ).
橱)逆转录聚合酶链反应 ( RT—PCR ) 方法研究大鼠心肌Gsa蛋白、Gia蛋白mRNA的表达.
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Methods: Mainly with methods of in situ reverse transcription polymerase chain reaction ( In situ RT - PCR ).
方法以原位逆转录聚合酶链式反应 ( Insitureverse transcriptionpolymerasechainreaction,InsituRT -PCR ) 为主要研究方法.
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TAUT and CSD mRNA levels were assayed using competitive quantitative reverse transcription polymerase chain reaction ( RT - PCR ).
竞争性RT-PCR测定TAUT和CSDmRNA的含量.
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The expressions of Slit 2 were tested by reverse transcription polymerase chain reaction ( RT - PCR ) and immunohistochemical measurement.
分别通过逆转录聚合酶链反应法 ( RT -PCR ) 和免疫组织化学方法检测Slit2基因的表达水平及部位.
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Expression of aquaporin 1 in renal tissue was observed by using real reverse transcription polymerase chain reaction.
实时逆转录聚合酶链式反应检测肾脏组织中水通道蛋白1表达.
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The proto - oncogene c - fos mRNA expression was assessed using reverse transcription polymerase chain reaction ( RT - PCR ).
用逆转录聚合酶链式反应 ( RT -PCR ) 检测心肌细胞原癌基因c-fosmRNA的表达.
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The gene expression of a - actin mRNA were determined by using the quantitative reverse transcription PCR technology.
利用半定量反转录聚合酶链式反应(QRT-PCR)法测定骨骼肌α-actin基因表达.
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