Objective : To find a rapid method of screening positive recombinant plasmid.

目的: 建立一种快速筛选阳性重组质粒的方法.

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Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.

构建所得的重组质粒可用于下游的转基因工作.

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Objective Construct a recombinant plasmid pET 28 a - EDA - EDB , prepare the fusion EDA - EDB protein.

目的构建重组pET28a-EDA-EDB 质粒, 制备重组纤维连接蛋白EDA -EDB 融合蛋白.

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Figure 2 . Restriction pattern of the recombinant plasmid.

图2. 阳性重组子质粒酶切电泳图谱.

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Results: The recombinant plasmid pUC 18 E 6 had been got.

结果: 获得重组质粒pUC18E6.

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The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.

从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃ 培养得不到转化子.

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A recombinant plasmid pUC - IL 6 coding for IL 6 was constructed by recombinant gene technique.

利用基因重组技术,构建含IL6基因的重组质粒pUC - IL6.

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Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

目的:消减文库构建过程中,用PCR技术快速筛选重组阳性克隆.

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Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3 - AMG in COS - 1 cell line.

目的研究人釉原蛋白(AMG) 重组质粒PcDNA3-AMG在COS -1 细胞系中的表达.

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Method The recombinant plasmid pMD 18 - T - gG was constructed as HSV - 2 DNA standard for quantitative analysis.

方法构建重组质粒pMD18-T-gG作为标准品.

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The recombinant plasmid structure was verified by double - enzyme cleavage and gene sequence analysis.

该质粒经双酶切鉴定、目的基因序列分析,证实了重组质粒结构的正确性.

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Methods: Transform COS 7 cells with recombinant plasmid and detect instantaneously expressed product by ELISA.

方法　质粒转染COS7细胞,用ELISA法测定瞬时表达产物.

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Results The Arresten gene was screening successfully, and the recombinant plasmid pBV 220 - Arr was constructed successful.

结果成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达.

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Recombinant plasmid pDAlllS was constructed by inserting 1.7 kb aprA into the Nrul site of aveD.

将1.7kb安普霉素抗性基因aprA插入aveD的 NruI位点,得pDA1118.

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