Methods: Cell culture in serum - free medium , indirect immunofluorescence cytochemistry were used.
方法: 采用无血清细胞培养技术, 间接免疫细胞化学染色法.
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CONCLUSION: The keratinocytes growed best in serum - free medium supplemented with EGF and BPE.
结论: 细胞在加入表皮生长因子和促进增殖的垂体提取物的无血清培养基中增殖良好.
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